I think it's 100% U235, otherwise it would read just "uranium" and would represent the energy density of a mix of U235 and U238. This comic is drawn by Randall Munroe, a former NASA guy. He tends to get the science right.
Wikipedia has the energy density of U235 at 83,140,000 MJ/KJ, so the comic was apparently being conservative.
Most US Powerplants (Pressurized Water Reactors) run at low enrichment (say around 4%). Submarine reactors are really the only reactors that run with very high U-235 enrichment levels for a number of reasons. Spot price is typically quoted for natural uranium.